cat. # BLS08-36-00001
5x HOT FIREPol® EvaGreen® qPCR Supermix
1ml | 250 rxn
Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipettingOptimized ready-to-use solution for dye-based real-time quantitative
PCR assays. This master mix has been developed to give highly specific and sensitive results, and demonstrates excellent performance with GC-rich regions.
· high sensitivity with low DNA concentrations
5x HOT FIREPol® EvaGreen® qPCR Supermix is an optimized 5X reaction mix for dye-based qPCR detection and quantitation of target DNA sequences
using EvaGreen® dye and SYBR®/FAM channel of most real-time qPCR instruments.
The Mix contains HOT FIREPol® DNA Polymerase and an passive internal reference dye that is compatible with most qPCR cyclers including high ROX or low ROX reference signal requiering platforms.
The Mix contains dUTPs to prevent cross-contamination with UNG treatment and visible dye to ease reaction set-up. This dye does not interfere with
· Detection and quantification of DNA and cDNA targets
Hot-start: yes, initial activation in 12 min
Detection type: dye-based, includes EvaGreen® intercalating dye
Reference dye: based on ROX
Compatible real-time instruments: most qPCR cyclers. Check Your cycler!
■ Mix Components
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs.
Internal reference based on ROX dye
Blue visualisation dye
■ EvaGreen Dye
EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is an extremely stable dye and has been shown to be non-mutagenic and non-cytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!
■ Trustworthy performance
■ Storage & Shipping
Routine storage: -20℃
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR Supermix.
At room temperature